Hemolytic Properties of Eastern and Western Equine Encephalomyelitis Viruses

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Abstract
Summary: Genetron-treated fluids of chick embryo cultures infected with Eastern and Western equine encephalomyelitis viruses were found, after freezing and thawing, to possess hemolytic activity over a wide temperature range (4 to 37°C). The virus hemolysin and hemagglutinin were inactivated by heat, whereas treatment with trypsin, ether and Ca++ tended to cause a selective suppression of the hemolysin activity of both viruses. Certain changes in the pH at which assays were performed resulted in a reduction of hemolytic activity which was greater than any change in hemagglutinating activity. Treatment of chick red blood cells with RDE and trypsin failed to remove the receptors for either of the activities. However, trypsin treatment significantly increased the hemagglutinating activity of both viruses. The hemolysins were inhibited by specific viral antibody and were sedimentable with the hemagglutinins.

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