Primary sequence of Xenopus laevis Na+-K+-ATPase and its localization in A6 kidney cells
- 1 June 1989
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Renal Physiology
- Vol. 256 (6) , F1034-F1043
- https://doi.org/10.1152/ajprenal.1989.256.6.f1034
Abstract
Polyclonal antibodies raised against the alpha- and beta-subunits of amphibian kidney Na+-K+-ATPase were used to screen an expression library from Xenopus laevis kidney epithelial cells (A6 cell line). cDNAs coding for each Na+-K+-ATPase subunit were identified and used to isolate near full-length cDNAs. The complete nucleotide sequence and the deduced amino acid sequence were determined. The alpha-subunit is an alpha (alpha I)-isoform. The alpha- and beta-subunits are more closely related to the mammalian and avian than the fish sequences. Antibodies raised against the fusion proteins produced by the two clones served to immunoprecipitate proteins from biosynthetically labeled or selectively surface-radioiodinated A6 cells grown on a porous substrate. The alpha- and the beta-subunits of Na+-K+-ATPase were found associated early in the course of biosynthesis and were restricted to the basolateral plasma membrane.This publication has 38 references indexed in Scilit:
- Immunochemical evidence for a transmembrane orientation of both the (sodium(1+), potassium(1+) ion-activated)-ATPase subunitsBiochemistry, 1981
- Transformation of E. coli using homopolymer-linked plasmid chimerasBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1981
- Apical membrane aminopeptidase appears at site of cell-cell contact in cultured kidney epithelial cells.Proceedings of the National Academy of Sciences, 1980
- Two molecular forms of (Na+ + K+)-stimulated ATPase in brain. Separation, and difference in affinity for strophanthidin.Journal of Biological Chemistry, 1979
- Cloning and characterization of synthetic sequences from the Xenopus laevis vitellogenin structural geneDevelopmental Biology, 1978
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- Purification and characterization of (Na+ + K+)-ATPase. VI. Differential tryptic modification of catalytic functions of the purified enzyme in presence of NaCl and KClBiochimica et Biophysica Acta (BBA) - Biomembranes, 1977
- Albumin Phylogeny for Clawed Frogs ( Xenopus )Science, 1977
- DNA content in the genus XenopusChromosoma, 1977
- Improved procedures for immunoferritin labeling of ultrathin frozen sections.The Journal of cell biology, 1976