Isolation of tobacco SSU genes: characterization of a transcriptionally active pseudogene
Open Access
- 1 January 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (21) , 8661-8677
- https://doi.org/10.1093/nar/15.21.8661
Abstract
Genomic clones containing three genes for the small subunit (SSU) of ribulose bisphosphate carboxylase were isolated from tobacco. Detailed analysis was performed on two of these clones to give a clearer picture of this multigene family in tobacco. This analysis demonstrated that one of the clones contained a pseudogene that was unusual in that it was transcriptionally active. This is the first transcriptionally active pseudogene that has been reported in plants. In addition, another clone was found to contain coding sequences which are 100% homologous to a previously-cloned tobacco SSU gene (Mazur, B.J. and Chiu, C-F. [1985] Nuc. Acids Res. 13, 2372–2386), indicating that gene duplication and/or gene conversion may have played a role in the evolution of the tobacco SSU family.Keywords
This publication has 37 references indexed in Scilit:
- Organ-Specific and Light-Induced Expression of Plant GenesScience, 1986
- Light regulation of plant gene expression by an upstream enhancer-like elementNature, 1985
- Molecular characterization and genetic mapping of two clusters of genes encoding chlorophyll a/b-binding proteins in Lycopersicon esculentum (tomato)Gene, 1985
- Phytochrome Control of the Expression of Two Nuclear Genes Encoding Chloroplast Proteins in Lemna gibba L. G-3Plant Physiology, 1983
- Recent divergence from a common ancestor of human IFN-α genesNature, 1982
- A transcriptionally active pseudogene in Xenopus laevis oocyte 5S DNACell, 1981
- The structure of eight distinct cloned human leukocyte interferon cDNAsNature, 1981
- Screening λgt Recombinant Clones by Hybridization to Single Plaques in SituScience, 1977
- Origin of Nicotiana tabacum L. detected by polypeptide composition of Fraction I proteinNature, 1974
- Host specificity of DNA produced by Escherichia coli: Bacterial mutations affecting the restriction and modification of DNAJournal of Molecular Biology, 1966