Cytokine Expression of Human Oral Keratinocytes

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Abstract
The present study aimed to examine the cytokine expression pattern of human oral mucosa-derived keratinocytes at the protein and RNA level. The mRNA expression was measured by a RT-PCR method and the protein production was determined by an ELISA technique. In freshly isolated oral keratinocytes, IL-1α (interleukin 1α), IL-6, IL-8, transforming growth factor β, tumor necrosis factor α and basic fibroblast growth factor were detectable at the protein and mRNA level, whereas platelet-derived growth factor, acidic fibroblast growth factor and transforming growth factor α were found only at the mRNA level. There were no detectable signals of IL-2 and IL-4. The cytokine production at the protein level was independent of prior stimulation with phorbol myristate acetate. Our results show that human oral keratinocytes – under nonpathological conditions – produce a cytokine pattern quite similar to that of epidermal keratinocytes, which may have implications for further functional studies.

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