Measurement of microvascular dimensions in vivo.

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Abstract
The operating characteristics of a flying-spot microscope designed for measurement of small blood vessels were examined. In studies of cat mesentery the device measured the width of the red cell column and did not pick up the walls of arterioles or venules. The flying-spot microscope did not detect changes in width of the red cell stream in a cylindrical channel as flow rate was varied. Also, the measured width did not change when hemolyzed blood was substituted for whole blood. The red cell column extends to the inside wall of the tube. Therefore, the flying-spot device can be used for measurement of internal diameter of blood vessels.