Enzymatic enantioselective C–C‐bond formation in microreactors
- 25 January 2008
- journal article
- other
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 99 (4) , 1028-1033
- https://doi.org/10.1002/bit.21649
Abstract
We have demonstrated that multiple crude enzyme lysates containing a hydroxynitrile lyase can be used for the enantioselective synthesis of cyanohydrins from aldehydes in microchannels. Using a microreactor setup, two important parameters were efficiently screened consuming only minute amounts of reagents. More importantly, results from the continuous flow reaction were fully consistent with results obtained from larger batchwise processes in which a stable emulsion was formed. Biotechnol. Bioeng. 2008; 99; 1028–1033.Keywords
This publication has 19 references indexed in Scilit:
- Microreactors as Tools for Synthetic Chemists—The Chemists' Round‐Bottomed Flask of the 21st Century?Chemistry – A European Journal, 2006
- Recent advances in synthetic micro reaction technologyChemical Communications, 2006
- Enantioselective Catalysis and Analysis on a ChipAngewandte Chemie International Edition in English, 2006
- Immobilization of enzymes on a microchannel surface through cross-linking polymerizationChemical Communications, 2005
- Enzymatic microreactors in chemical analysis and kinetic studiesBiotechnology Advances, 2005
- Enhancement of Chemical Selectivity by MicroreactorsChemical Engineering & Technology, 2005
- Microreactor-based reaction optimization in organic chemistry—glycosylation as a challengeChemical Communications, 2004
- Comprehensive Step‐by‐Step Engineering of an (R)‐Hydroxynitrile Lyase for Large‐Scale Asymmetric SynthesisAngewandte Chemie International Edition in English, 2003
- Rapid enzymatic transglycosylation and oligosaccharide synthesis in a microchip reactorLab on a Chip, 2002
- Enhanced Enzymatic Reactions in a Microchannel ReactorAustralian Journal of Chemistry, 2002