A simple and rapid method for preparing genomic DNA from gram-positive bacteria
- 1 October 1998
- journal article
- Published by Springer Nature in Molecular Biotechnology
- Vol. 10 (2) , 191-193
- https://doi.org/10.1007/bf02760866
Abstract
Molecular epidemiologic and other studies may require preparation of genomic DNA from large numbers of bacteria in sufficiently pure form for restriction endonuclease digestion, cloning, RAPD-PCR, Southern hybridization, and so on.Staphylococcus and other Gram-positive bacteria have a rigid cell wall and can be difficult to lyse. Here, a simple and rapid method for the preparation of genomic DNA from multiple samples is reported. This method produces clean DNA for use in most molecular biology methods in <90 min.Keywords
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