POLYSACCHARIDE COATING OF HUMAN CORNEAL ENDOTHELIUM

Abstract
Electron microscopy revealed the presence of a 600–1500 å thick layer of polysaccharide on the surface of human corneal endothelial cells. The surface layer was visualized by combined fixation and staining in a mixture of ruthenium red and osmium tetroxide. The coating material was stable for at least 39 h post mortem and was retained on disintegrating cells.