Coumarin–phalloidin: A new actin probe permitting triple immunofluorescence microscopy of the cytoskeleton
Open Access
- 1 January 1988
- journal article
- research article
- Published by The Company of Biologists in Journal of Cell Science
- Vol. 89 (1) , 21-24
- https://doi.org/10.1242/jcs.89.1.21
Abstract
7-Diethylamino-3-(4-isothiocyanotophenyl)-4-methylcoumarin (CPITC) was coupled to amino-methyldithiolanophalloidin to produce a new phalloidin derivative, coumarin–phalloidin, fluorescent in the blue region of the spectrum. Coumarin–phalloidin binds to actin with around 100-fold less affinity than unconjugated phalloidin, but with enough avidity to make it a useful stain for actin filaments. Appropriate filter combinations permit triple immunofluorescence microscopy of the cytoskeleton with fluorescein and rhodamine conjugates together with coumarin-phalloidin.This publication has 16 references indexed in Scilit:
- Fading of immunofluorescence during microscopy: a study of the phenomenon and its remedyPublished by Elsevier ,2002
- Factors Regulating the Presence of Microtubules in CellsAnnals of the New York Academy of Sciences, 1986
- Localization of filamin in smooth muscle.The Journal of cell biology, 1986
- Preparation of tetramethylrhodaminyl-phalloidin and uptake of the toxin into short-term cultured hepatocytes by endocytosisExperimental Cell Research, 1983
- Analogs of phalloidinInternational Journal of Peptide and Protein Research, 1983
- Fluorescence Microscopy: Reduced Photobleaching of Rhodamine and Fluorescein Protein Conjugates by n -Propyl GallateScience, 1982
- New fluorochromes for thiols: maleimide and iodoacetamide derivatives of a 3-phenylcoumarin fluorophore.Journal of Histochemistry & Cytochemistry, 1981
- Fluorescent staining of microfilaments with heavy meromyosin labeled with N-(7-dimethylamino-4-methylcoumarinyl) maleimide.Journal of Histochemistry & Cytochemistry, 1980
- über die Inhaltsstoffe des grünen Knollenblätterpilzes, LVIII. Einige vom Ketophalloidin abgeleitete, in der biochemischen Forschung anwendbare DithiolaneEuropean Journal of Organic Chemistry, 1980
- Preparation of a semipermanent mounting medium for fluorescent antibody studiesVirology, 1960