AFFINITY LABELING OF CAMP-DEPENDENT PROTEIN-KINASE WITH PARA-FLUOROSULFONYLBENZOYL ADENOSINE - COVALENT MODIFICATION OF LYSINE 71
- 1 January 1981
- journal article
- research article
- Vol. 256 (21) , 837-842
Abstract
P-Fluorosulfonylbenzoyl 5''-adenosine (FSO2BzAdo) was shown previously to be an irreversible inhibitor of the catalytic subunit of cAMP-dependent protein kinase II from porcine skeletal muscle. The catalytic subunit of porcine heart cAMP-dependent protein kinase was also inhibited following incubation with FSO2[14C]BzAdo and inhibition was shown to result from the stoichiometric, covalent modification of a single lysine residue. The amino acid sequence in an extended region around the carboxybenzenesulfonyl lysine (CBS-lysine) was elucidated by characterizing both tryptic and cyanogen bromide peptides containing the 14C-modified residue. The sequence in this region was Leu-Val-Lys-His-Lys-Glu-Thr-Gly-Asn-His-Phe-Ala-Met-Lys(CBS)-Ile-Leu-Asp-Lys-Glu-Lys-Val-Val-Lys-Leu-Lys-Gln-Ile. The covalently modified residue corresponded to lysine 71 in the overall polypeptide chain. Homologies to bovine heart catalytic subunit and to a site modified by FSO2BzAdo in phosphofructokinase are considered.This publication has 22 references indexed in Scilit:
- Identification of a tyrosine residue at a nucleotide binding site in the beta subunit of the mitochondrial ATPase with p-fluorosulfonyl[14C]-benzoyl-5'-adenosine.Journal of Biological Chemistry, 1978
- Affinity labeling of AMP-ADP sites in heart phosphofructokinase by 5′-p-fluorosulfonylbenzoyl adenosineBiochemical and Biophysical Research Communications, 1978
- A procedure for the synthesis of p-fluorosulfonyl[14C]-benzoyl-5′-adenosine with [14C] in the benzoyl moietyAnalytical Biochemistry, 1978
- Methanol solvent system for rapid analysis of phenylthiohydantoin amino acids by high-pressure liquid chromatographyJournal of Chromatography A, 1978
- Adenosine 3':5'-monophosphate dependent protein kinase from bovine heart. Characterization of the catalytic subunitBiochemistry, 1977
- Role of multiple basic residues in determining the substrate specificity of cyclic AMP-dependent protein kinase.Journal of Biological Chemistry, 1977
- Affinity labeling of rabbit muscle pyruvate kinase by 5'-p-fluorosulfonylbenzoyladenosineBiochemistry, 1977
- Purification and characterization of catalytic subunit of skeletal muscle adenosine 3':5'-monophosphate-dependent protein kinase.Journal of Biological Chemistry, 1977
- Purification and characterization of the catalytic subunit of adenosine 3':5'-cyclic monophosphate-dependent protein kinase from bovine liverBiochemical Journal, 1976
- THE OXIDATION OF RIBONUCLEASE WITH PERFORMIC ACIDJournal of Biological Chemistry, 1956