Periodic DNA synthesis in cell-free extracts of Xenopus eggs.
Open Access
- 1 July 1987
- journal article
- research article
- Published by Springer Nature in The EMBO Journal
- Vol. 6 (7) , 2003-2010
- https://doi.org/10.1002/j.1460-2075.1987.tb02464.x
Abstract
Cell‐free extracts prepared from unfertilized eggs of Xenopus laevis support DNA synthesis on sperm pronuclei. Continuous labelling studies using [3H]dCTP and pulse labelling studies using [32P]dCTP demonstrate that synthesis occurs in short bursts of 40 min, which are punctuated by periods of 20‐40 min during which no synthesis occurs. Density substitution experiments using bromodeoxyuridine demonstrate that this synthesis involves the initiation of replication and reveals that re‐initiation events can occur following multiple bursts of replication. The periodic properties of these extracts are sensitive to protein synthesis inhibitors.This publication has 20 references indexed in Scilit:
- Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggsPublished by Elsevier ,1986
- Induction of early mitotic events in a cell-free systemCell, 1985
- Lack of specific sequence requirement for DNA replication in Xenopus eggs compared with high sequence specificity in yeastCell, 1984
- Tumor promoter TPA increases initiation of replication on DNA injected into Xenopus eggsCell, 1983
- Formation in Vitro of Sperm Pronuclei and Mitotic Chromosomes Induced by Amphibian Ooplasmic ComponentsScience, 1983
- Regulated replication of DNA microinjected into eggs of Xenopus laevisCell, 1980
- Eucaryotic DNA: Organization of the genome for replicationCell, 1978
- EUKARYOTIC CHROMOSOME REPLICATIONAnnual Review of Genetics, 1975
- DNA synthesis in oocytes and eggs of Xenopus laevis injected with DNADevelopmental Biology, 1975
- Induction of Polyoma DNA Synthesis by Injection into Frog‐Egg CytoplasmEuropean Journal of Biochemistry, 1973