Intensification of Osmium Staining byp-Phenylenediamine: Paraffin and Epon Embedding; Lipid Granules in Renal Medulla
- 1 January 1970
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 45 (6) , 255-260
- https://doi.org/10.3109/10520297009067797
Abstract
Tissue which had been fixed in 4% glutaraldehyde and postfixed in 2% OsO4 was subsequently treated with p-phenylenediamine, either in the block prior to embedding in paraffin or Epon or, in the case of Epon-embedded material, after sectioning for light microscopy. The p-phenylenediamine was best used as a 0.8-1% solution in 70% alcohol. The p-phenylenediamine caused a very considerable intensification of staining of any cell components; this intensification of staining was particularly marked in the case of the lipid granules of renal medulla.Keywords
This publication has 1 reference indexed in Scilit:
- IMPROVEMENTS IN EPOXY RESIN EMBEDDING METHODSThe Journal of cell biology, 1961