The highly related DEAD box RNA helicases p68 and p72 exist as heterodimers in cells
- 1 March 2003
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 31 (5) , 1470-1480
- https://doi.org/10.1093/nar/gkg236
Abstract
The RNA helicases p68 and p72 are highly related members of the DEAD box family of proteins, sharing 90% identity across the conserved core, and have been shown to be involved in both transcription and mRNA processing. We previously showed that these proteins co-localise in the nucleus of interphase cells. In this study we show that p68 and p72 can interact with each other and self-associate in the yeast two-hybrid system. Co-immunoprecipitation experiments confirmed that p68 and p72 can interact in the cell and indicated that these proteins preferentially exist as hetero-dimers. In addition, we show that p68 can interact with NFAR-2, a protein that is also thought to function in mRNA processing. Moreover, gel filtration analysis suggests that p68 and p72 can exist in a variety of complexes in the cell (ranging from approximately 150 to approximately 400 kDa in size), with a subset of p68 molecules being in very large complexes (>2 MDa). The potential to exist in different complexes that may contain p68 and/or p72, together with a range of other factors, would provide the potential for these proteins to interact with different RNA substrates and would be consistent with recent reports implying a wide range of functions for p68/p72.Keywords
This publication has 37 references indexed in Scilit:
- RH70, a Bidirectional RNA Helicase, Co-purifies with U1snRNPPublished by Elsevier ,2002
- Regulation of Alternative Splicing by the ATP-Dependent DEAD-Box RNA Helicase p72Molecular and Cellular Biology, 2002
- The mRNA of DEAD Box Protein p72 Is Alternatively Translated into an 82-kDa RNA HelicaseJournal of Biological Chemistry, 2002
- Overexpression and poly-ubiquitylation of the DEAD-box RNA helicase p68 in colorectal tumoursOncogene, 2001
- Expression of p68 RNA Helicase Is Closely Related to the Early Stage of Adipocyte Differentiation of Mouse 3T3-L1 CellsBiochemical and Biophysical Research Communications, 2001
- A subfamily of RNA-binding DEAD-box proteins acts as an estrogen receptor α coactivator through the N-terminal activation domain (AF-1) with an RNA coactivator, SRAThe EMBO Journal, 2001
- The Nuclear DEAD Box RNA Helicase p68 Interacts with the Nucleolar Protein Fibrillarin and Colocalizes Specifically in Nascent Nucleoli during TelophaseExperimental Cell Research, 2000
- High-level expression in male germ cells of murine P68 RNA helicase mRNALife Sciences, 1993
- RNA helicase activity associated with the human p68 proteinNature, 1989
- Nuclear protein with sequence homology to translation initiation factor eIF-4ANature, 1988