Renin inactivation by human plasma

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Abstract
To study the inactivation of renin by human plasma, human angiotensinogen was partially purified by the method of Haas et al. (1966) and human renin was separated from angiotensinases by passing the kidney extract through a carboxymethyl cellulose column (CMC). Partially purified human angiotensinogen is almost free of angiotensinase activity when used in diluted solutions and for short periods of incubation. In concentrated solution, a residual angiotensinase activity is observed, which belongs probably to angiotensinase B. CMC chromatography of kidney extracts separates renin from angiotensinases and gives a recovery higher than 50% for renin. The renin obtained with this method is stable at 0° for 24 h, while when incubated in the presence of human plasma at 37 °C for 2 or 4 h, its activity decreases significantly. The renin-inactivating factor present in human plasma destroys rat renin, while it does not modify the activity of hog renin. The renin inactivation is prevented when plasma is treated with EDTA (20 mM) or acidified at pH 3.0 for 30 min before incubation. The renin inactivating system, present in human plasma, is different from renal angiotensinases and may be an enzyme.

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