Repression of in vitro transcription of the Escherichia coli fnr and nar X genes by FNR protein
Open Access
- 28 February 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 340 (1-2) , 59-64
- https://doi.org/10.1016/0014-5793(94)80173-8
Abstract
In facultative anaerobes, the anaerobic expression of respiratory genes is regulated by a transcriptional activator, FNR. Transcription in vitro of the E. coli fnr gene was repressed by its product, FNR. The transcription of the E. coli nar X gene encoding the nitrate sensor protein was likewise repressed. DNA truncation experiments for fnr and nar X genes indicated that multiple anaero‐boxes in each promoter region are essential for repression by the FNR protein, but they also suggest that factor‐independent upstream activation signals are operating with these promoters.Keywords
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