Abstract
Experiments were conducted on juvenile American shad,Alosa sapidissima, to indicate tolerance to reduced oxygen, toxicity of petroleum products, and toxicity of petroleum products with accompanying low dissolved oxygen. All mortalities in reduced oxygen tests occurred at dissolved oxygen values of less than 2 ppm with lethal concentrations varying with the rate of reduction. No mortalities occurred when dissolved oxygen was maintained between 2 and 4 ppm. TLm (median tolerance limit) values were determined for gasoline, diesel fuel oil, and bunker oil for 24-and 48-hour periods. Gasoline was the most toxic, fuel oil somewhat less toxic, and bunker oil least toxic. The lethality of petroleum products to shad was found to increase when accompanied by low dissolved oxygen.

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