Poliovirus-specific Polypeptides in Infected HeLa Cells Analysed by Isoelectric Focusing and 2D-Analysis
- 1 January 1981
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 52 (1) , 61-69
- https://doi.org/10.1099/0022-1317-52-1-61
Abstract
Analysis of poliovirus-infected [human cervical carcinoma] cells by isoelectric focusing in urea resolves at least 25 bands. Combination of isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in a 2-D analysis revealed a much better resolution than each method separately. By 2D-analysis most of the bands could be correlated with the known poliovirus-specific polypeptides generated in the infected cell. With this technique it was possible to determine the apparent isoelectric point (pI) of the identified poliovirus polypeptides. They are in the range from pH 5.6 for polypeptide 7d to pH 8.8 for polypeptide X, which is the most basic found so far.This publication has 5 references indexed in Scilit:
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- Isoelectric focusing and 2D-analysis of poliovirus proteinsVirology, 1977
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