Quantitative Analysis of the Twenty Natural Protein Amino Acids by Gas-Liquid Chromatography

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Abstract
A quantitative gas-chromatographic method has been developed and kipplied to the analysis of the 20 protein amino acids and other nonprotein amino acids as their Ntrifluoroacety | nbuty| esters. Pure reference standards were prepared and used to determine yield of derivatives. Yields of 98 ≠ 3% were obtained for dl the amino acids. The calibration curves were linear and showed no statistical bias. Results obtained from malysis of amino acids mixtures and proteins by gas chromatography were in excellent agreement with theory and ionexchange. For qnantitative analysis the amount of amino acid injected is 0.05 to 35μg. The relative standard deviations range from 0.66 to 2.28%. Qualitative analyses can be made on 1 to 10 ng of amino acid injected. The merits and applicability of the relative mole per cent, internal standard, and slope factor methods of calculation ofthe percentage of amino acids are presented and discussed. This method has broad implications in advancing studies in genetics, protein biochemistry, agricultural sciences, medical research, and nutrition.