THE HETEROGENEITY OF MONONUCLEAR PHAGOCYTES IN LYMPHOID ORGANS - DISTINCT MACROPHAGE SUBPOPULATIONS IN THE RAT RECOGNIZED BY MONOCLONAL ANTIBODY-ED1, ANTIBODY-ED2 AND ANTIBODY-ED3

  • 1 January 1985
    • journal article
    • research article
    • Vol. 54  (3) , 589-599
Abstract
In the present study, a set of 3 monoclonal antibodies is described, each of which recognizes cells of the monocyte-macrophage lineage in the rat. The tissue distribution, in particular in lymphoid organs, of each of the 3 monoclonals is determined by immunoenzyme histochemistry on cryostate sections, as well as on cell suspensions. Results show that ED1 recognizes a cytoplasmic antigen in monocytes and in most macrophages, free and fixed. ED2 and ED3 recognize membrane antigens of tissue macrophages, discriminating between distinct subpopulations of macrophages, each with a characteristic localization in the compartments of lymphoid organs. No other cell types except cells of the mononuclear phagocyte system are positive for any of the 3 monoclonals. Possible relations between the macrophages recognized by this set of monoclonals and dendritic cells are discussed.

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