Effect of Conditioning Solvent on the Orientation of Bonded Hydrocarbon Moieties in Totally Aqueous Mobile Phases

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Abstract
The effect of various organic conditioning solvents on the orientation of a bonded decyl surface, in combination with water as the mobile phase, was studied. Observed changes in the thermal dependency of solute retention measured on the initially formed, collapsed, or folded state were related to size, shape, and polarity of the organic conditioning solvent used. These data are explained in terms of an entrapment model in which solvent is incorporated into the bonded hydrocarbon layer during formation of the collapsed state, after an abrupt change from organic to toally aqueous conditions. Further, the entrapment and release of solvent were found to occur by a two-step process: an initial rapid release followed by a gradual secondary release that persisted over many hundreds of column volumes. The extended time for the secondary release of solvent is interpreted as a suggestion that the incorporation of conditioning solvent into the initially formed, collapsed or folded state is via hydrocarbon clustering mechanism.

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