The Determination of alpha-Tocopherol in Animal Tissues by Column Chromatography.
- 1 January 1961
- journal article
- research article
- Published by Danish Chemical Society in Acta Chemica Scandinavica
- Vol. 15 (4) , 783-790
- https://doi.org/10.3891/acta.chem.scand.15-0783
Abstract
A rapid method for the chromatographic separation and quantitative determination of small amounts of [alpha]-tocopherol in the unsaponifiable matter of animal tissue is described. The unsaponifiable material from 0.5 [long dash]1.5 g of tissue, containing 1[long dash]20 [mu]g of [alpha]-tocopherol, is put on a 0.5 x 5 cm column packed with a mixture of activated alumina, zinc carbonate and Celite. Twenty per cent benzene in cyclohexane is passed through the column and eight fractions of 1 ml are collected. The Emmerie-Engel reaction is performed on each fraction. [alpha]-Toco-pherol separates sharply in 3[long dash]4 fractions free of other reducing substances.This publication has 1 reference indexed in Scilit:
- Effect of Coenzyme Q10 on the Determination of Tocopherol in Animal TissueJournal of Biological Chemistry, 1960