The binding of colonization factor antigens of enterotoxigenic Escherichia coli to intestinal cell membrane proteins

1 January 1990

journal article
Published by Oxford University Press (OUP) in FEMS Microbiology Letters

Vol. 66 (1-3) , 107-112
https://doi.org/10.1016/0378-1097(90)90266-s

Abstract

We examined the binding of colonization factor antigens (CFAs) of enterotoxigenic Escherichia coli to electrophoretically separated membrane components of rabbit intestinal brush borders or human intestinal (and non-intestinal) cell lines using an immunoblotting technique. Both CFA/I and CFA/II bound to distinct membrane components which seemed to be identical in rabbit brush borders and in a human intestinal cell line; these binding structures were mainly missing in membranes from epithelial cell lines of non-intestinal origin. Both shared and specific binding components were identified for CFA/I and the different subcomponents of CFA/II (CS1, CS2 and CS3), respectively. Chloroform-methanol extraction of lipids from the cell membranes did not change the binding pattern for either CFA/I or CFA/II suggesting that the binding occurred to (glyco)proteins rather than to (glyco)lipids.

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