Hydrolysis of Mixed Monomolecular Filme of phosphatidulchoiline/Triacylglycerol by Pancereatic Phospholipase A2
- 1 May 1983
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 132 (3) , 639-644
- https://doi.org/10.1111/j.1432-1033.1983.tb07411.x
Abstract
We studied the effect of glycerides on pancreatic phospholopase A2 hydrolysis of mixed monomoecular films of trioctanoylglyderol/1, 2‐didodecanoyl‐sn‐ glycero‐3‐phosphocholine with the technique of Piéroni and Verger [(1979) J. Biol. Chem. 254, 10090–10094]. The quantity of enzyme adsorbed to the interface was concomitantly determined with [3H] amidinated phospholipase. At phospholipid packing above the critical penetration pressure, triacylglycerol stimulates phosphatidylcholone hydrolysis to a great extent. On th eother hans, the activity of pancreatic phospholipase A2 on a mixed film is inhibited by the action o fpandreatic lipase. Interface binding of phospholipase A2 to the lipid sustrate does not imply activity.This publication has 42 references indexed in Scilit:
- Effect of Taurodeoxycholate, Colipase and Temperature on the Interfacial Inactivation of Porcine Pancreatic LipaseEuropean Journal of Biochemistry, 1980
- Action of phospholipase A2 on unmodified phosphatidylcholine bilayers: Organizational defects are preferred sites of actionThe Journal of Membrane Biology, 1980
- Lipoprotein Lipase: Some Effects of Activator ProteinsEuropean Journal of Biochemistry, 1980
- The influence of bile salts and bile lipoprotein complex on pancreatic lipase hydrolysis of monomolecular filmsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1980
- Correlation between the thermotropic behavior of sphingomyelin liposomes and sphingomyelin hydrolysis by sphingomyelinase of Staphylococcus aureusBiochimica et Biophysica Acta (BBA) - Biomembranes, 1978
- Specificity reversal in phospholipase A2 hydrolysis of lipid mixturesBiochemical and Biophysical Research Communications, 1978
- Membranes undergoing phase transitions are preferentially hydrolyzed by beta-bungarotoxinBiochimica et Biophysica Acta (BBA) - Biomembranes, 1977
- Effect of a diazafluoranthen derivative on phospholipases A study at the air-water interfaceBiochimica et Biophysica Acta (BBA) - Biomembranes, 1976
- Relation between various phospholipase actions on human red cell membranes and the interfacial phospholipid pressure in monolayersBiochimica et Biophysica Acta (BBA) - Biomembranes, 1975
- Studies on phospholipase A and its zymogen from porcine pancreas: III. Action of the enzyme on short-chain lecithinsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1971