Annotating genomes with massive-scale RNA sequencing
Open Access
- 16 December 2008
- journal article
- research article
- Published by Springer Nature in Genome Biology
- Vol. 9 (12) , R175
- https://doi.org/10.1186/gb-2008-9-12-r175
Abstract
Next generation technologies enable massive-scale cDNA sequencing (so-called RNA-Seq). Mainly because of the difficulty of aligning short reads on exon-exon junctions, no attempts have been made so far to use RNA-Seq for building gene models de novo, that is, in the absence of a set of known genes and/or splicing events. We present G-Mo.R-Se (Gene Modelling using RNA-Seq), an approach aimed at building gene models directly from RNA-Seq and demonstrate its utility on the grapevine genome.Keywords
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