Endonuclease Activities in Extracts ofMicrococcus Luteusthat Act on γ-irradiated DNA
- 1 January 1977
- journal article
- research article
- Published by Taylor & Francis in International Journal of Radiation Biology
- Vol. 31 (3) , 227-235
- https://doi.org/10.1080/09553007714550271
Abstract
Several protein fractions containing endonuclease activity against .gamma.-irradiated DNA (.gamma.-endonuclease) were isolated from M. luteus. The crude extract was eluted on a phosphocellulose column and chromatographed on TEAE cellulose and subsequently on hydroxyapatite. Peaks (5) of .gamma.-endonuclease were obtained from each preparation. Repeated experiments showed comparable chromatographic behavior of the fractions. There was no detectable activity of UV-endonuclease in the fractions with .gamma.-endonuclease but a small contamination of endonuclease against unirradiated DNA and against DNA with apurinic sites. The .gamma.-endonuclease is stimulated by, but is not dependent on, Mg. Several tests for endonuclease activity were used: the analysis of strand breaks in calf-thymus DNA or in [phage] PM2 DNA, and the determination of end-groups formed by endonuclease, either 3''OH end-groups or phosphomonoester end groups. The strand breaks induced by the .gamma.-endonuclease apparently carry 3''-OH and 5'' phosphate end groups.This publication has 22 references indexed in Scilit:
- Partial purification of endonuclease activity from human lymphoblasts Separation of activities for depurinated DNA and DNA irradiated with ultraviolet lightBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1975
- Purification and characterization of an endonuclease from calf thymus acting on irradiated DNABiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1975
- Correlation between sedimentation coefficient and molecular weight of denatured DNABiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1974
- The Enzymatic Release of O 6 -methylguanine and 3-methyladenine from DNA Reacted with the Carcinogen N -methyl- N -nitrosoureaProceedings of the National Academy of Sciences, 1974
- Action of DNA polymerase I on γ-irradiated DNABiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
- Action of exonuclease III on γ-irradiated DNABiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
- Enzymic repair of DNA. III. Properties of the uv-endonuclease and uv-exonucleaseBiochemistry, 1971
- Strahlenempfindlichkeit von Bakteriophagen-DNS /Radiation sensitivity of Bacteriophage DNAZeitschrift für Naturforschung B, 1969
- Enzymes Involved in the Early Stages of Repair of Ultraviolet-Irradiated DNAPublished by Cold Spring Harbor Laboratory ,1968
- An Improved Preparation of Sodium DesoxyribonucleateJournal of the American Chemical Society, 1952