Isolation and characterization of specialized transducing λ phages carrying ribosomal protein genes of Escherichia coli

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Abstract
Specialized transducing λ phages have been isolated which carry the regions of the Escherichia coli chromosome containing the gene or gene-clusters for ribosomal proteins (r-proteins) S 1, S 6-S 18-L 9, S 16-L 19, and L 28-L 33. To investigate whether these phages also carry the genes for r-proteins S 9, L 13, L 20, L 31, and L 34 whose gene locations are not known, cells irradiated with UV light were infected with these phages and the r-proteins synthesized were analyzed by two-dimensional gel electrophoresis. However, no synthesis of these r-proteins was stimulated, indicating that their genes are not located in the neighborhood of any of the above-mentioned genes or gene clusters. It was found that proteins S 6 and S 18 synthesized in the cells irradiated with UV light were not modified under these conditions in which no concomitant ribosome assembly took place.