Studies on the Mechanism of Estrogen Biosynthesis. VIII. The Development of Inhibitors of the Enzyme System in Human Placenta1
- 1 March 1973
- journal article
- other
- Published by The Endocrine Society in Endocrinology
- Vol. 92 (3) , 866-880
- https://doi.org/10.1210/endo-92-3-866
Abstract
Structural features of over 100 steroids and some non—steroids were evaluated systematically as inhibitors of estrogen biosynthesis in human placental microsomes. The microsomes were preincubated with and without potential inhibitors, and then were incubated with [4–14C] androst–4–ene–3,17–dione or [4–14C]3P—hydroxyandrost— S—en—17–one (DHA) and an NADPH generating system. After adding tritiated estrogens as recovery markers, the estrogens were isolated by thin—layer chromatography and were assayed using dual—label scintillation counting to determine percent conversion and inhibition produced. C10 A4–3–keto compounds were better inhibitors than the corresponding ring A reduced (5Endocrinology92: 866, 1973)Keywords
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