Effects of Indomethacin on Prostaglandin Synthesis and on Contractile Response of the Guinea Pig Gallbladder
- 1 January 1981
- journal article
- research article
- Published by S. Karger AG in Pharmacology
- Vol. 23 (2) , 95-101
- https://doi.org/10.1159/000137535
Abstract
In studies on prostaglandin (PG) synthetase activity in the guinea pig gallbladder, prostaglandin E2 and F2α (PGE2 and PGF2α) were enzymatically formed from arachidonic acid using the 105,000-g precipitate fraction. The amount of PGE2 synthesized was about four times larger than PGF2α. Indomethacin (1–3 µM) inhibited both the PG synthesis and the contractile response to transmural stimulation in the gallbladder. These findings suggest that the inhibitory effect of indomethacin on the contractility of the gallbladder is related to the inhibition of PG synthesis, and that endogenous PGE2 is involved in the contractility of this tissue.Keywords
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