Isolation of Human Clq as a by-Product of Routine Fractionation
- 1 January 1979
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 9 (3) , 281-291
- https://doi.org/10.1080/00327487908061692
Abstract
Clq can be isolated as a by-product of large scale fractionation. The euglobulins of fraction III are submitted to chromatography on CM-cellulose in a batch-procedure. The active fraction being frequently contaminated by lysozyme the protein can be further purified by gel-filtration in the presence of carbohydrates. The purified Clq is suitable for the detection of immune complexes.Keywords
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