Kinetics of acetylcholinesterase immobilized on polyethylene tubing

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Abstract
Acetylcholinesterase [from electric eel] was covalently attached to the inner surface of polyethylene tubing. Initial oxidation generated surface carboxylic groups which, on reaction with thionyl chloride, produced acid chloride groups; these were caused to react with excess ethylenediamine. The amine groups on the surface were linked to glutaraldehyde, and acetylcholinesterase was then attached to the surface. Various kinetic tests showed the catalysis of the hydrolysis of acetylthiocholine iodide to be diffusion controlled. The apparent Km is strongly dependent on flow rate and is much larger than the value for the free enzyme. Rate measurements over the temperature range 6-42.degree. C showed changes in activation energies consistent with diffusion control.

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