Detection of Murine Norovirus 1 by Using Plaque Assay, Transfection Assay, and Real-Time Reverse Transcription-PCR before and after Heat Exposure
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- 15 January 2008
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 74 (2) , 543-546
- https://doi.org/10.1128/aem.01039-07
Abstract
The correlation between the detection of murine norovirus 1 RNA by real-time reverse transcription-PCR and the infectivity by plaque assay before and after heat exposure (80 degrees C) was examined. No correlation was found in the current study. Moreover, heat inactivation had a much stronger detrimental effect on virus infectivity than on the integrity of the viral genome.Keywords
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