Absorption and Translocation of Ammonium 2,4-Dichlorophenoxyacetate by Bean Plants

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Abstract
Absorption of NH4(2,4-D).by young Red Kidney bean leaves was detd. (under various conditions) by a spectrophotometric method. Most of the absorption of the salt from an wholly aqueous soln. occurred within the first 4 hrs. after application. Addition of 0.5% by wt. of Carbowax 1500 to the soln. resulted in the growth-regulator being absorbed continuously during the period of treatment (72 hrs.). The arat. of NH4(2,4-D) absorbed was positively correlated with temp. values during treatment, both with and without Carbowax. There was no significant difference between the amts. of the salt absorbed at 100 ft.-candles of light and at 900 ft.-candles. The amount absorbed in the dark was significantly greater than in the light. All data indicated that no translocation of the compound from the treated leaf occurred in complete darkness. All evidence suggested that the rate of translocation of NH4-(2,4-D) was positively correlated with light intensity prevailing during treatment, at least for the lower light intensities. No effect of Carbowax 1500 on translocation of the salt was indicated. Data concerning the effects of temp. on the rate of translocation of the growth-regulator were inconclusive.

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