Incorporation and Toxicity of 32P‐Orthophosphate and Occurrence of Poly phosphate in Entamoeba Trophozoites1

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Abstract
The requirements of inorganic phosphate (Pi), the incorporation of 32P-orthophosphate (32Pi), and the occurrence of inorganic polyphosphate (polyP) in axenic Entamoeba cultures were explored. Maximal population densities and growth rates of E. histolytica trophozoites were attained in complete TP-S-1 medium. As 32Pi concentration was increased in the medium, its own incorporation and the culture growth rate were progressively inhibited, especially in Pi-deficient medium. PolyP grains were found in the cytoplasm and occasionally in the nuclear membrane of E. histolytica, E. histolytica-like, E. invadens and E. moshkovskii trophozoites.

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