D-Glyceraldehyde-3-Phosphate Dehydrogenase Amino-Acid Sequence of the Enzyme from the Extreme Thermophile Thermus aquaticus

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Abstract
The amino acid sequence of D-glyceraldehyde-3-phosphate dehydrogenase from the extreme thermophile T. aquaticus was elucidated. The polypeptide contains 332 amino acids and its sequence is 70% identical with that of the enzyme from the moderate thermophile Bacillus stearothermophilus. In contrast to less thermostable forms of the enzymes from B. stearothermophilus, pig, lobster and yeast, the T. aquaticus enzyme has only 1-cysteine residue, namely cysteine-149, which is required for catalysis.