The 245 base-pairoriCsequence of theE. colichromosome directs bidirectional replication at an adjacent region
Open Access
- 11 May 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 11 (9) , 2617-2626
- https://doi.org/10.1093/nar/11.9.2617
Abstract
The replication origin of the E.coli K-12 chromosome has been isolated as autonomously replicating molecules (oriC plasmid), and the DNA region essential for replicating function (oriC) has been localized to a sequence of 232–245 base-pairs(bp) by deletion analysis. In this report, the functional role of oriC was analysed by using an in vitro replication system and various OriC* and OniC− plasmids previously constructed. The results obtained were summarized as follows: (1) The oriC sequence contained information enough to direct bidirectional replication. (2) The actual DNA replication began at a region near, but outside, oriC and progressed bidirectionally. (3) Initiation of DNA synthesis at the specific region required the dnaA-complementing fraction from cells harboring a dnaA-carrying plasmid.Keywords
This publication has 12 references indexed in Scilit:
- An in vitro method generating base substitutions in preselected regions of plasmid DNA: Application to structural analysis of the replication origin of the Escherichia coli K-12 chromosomeGene, 1982
- Enzymatic replication of E. coli chromosomal origin is bidirectionalNature, 1982
- Enzymatic replication of the origin of the Escherichia coli chromosome.Proceedings of the National Academy of Sciences, 1981
- Promoters in the E. coli replication originNature, 1981
- Nucleotide sequence of Escherichia coli K-12 replication origin.Proceedings of the National Academy of Sciences, 1979
- Bacteriophage lambda carrying the Escherichia coli chromosomal region of the replication origin.Proceedings of the National Academy of Sciences, 1978
- Origin of replication, oriC, of the Escherichia coli chromosome on specialized transducing phages λasnMolecular Genetics and Genomics, 1978
- Cloning and mapping of the replication origin of Escherichia coli.Proceedings of the National Academy of Sciences, 1977
- Novel F prime factors able to replicate in Escherichia coli Hfr strains.Proceedings of the National Academy of Sciences, 1976
- Isolation and Partial Characterization of a Mutant of Escherichia coli Deficient in DNA Polymerase IIProceedings of the National Academy of Sciences, 1972