The Effect of Cyclic AMP on Catabolite Repression of Isocitrate Lyase in Nocardia salmonicolor (NCIB9701)
- 1 November 1976
- journal article
- research article
- Published by Microbiology Society in Journal of General Microbiology
- Vol. 97 (1) , 133-135
- https://doi.org/10.1099/00221287-97-1-133
Abstract
Adding sodium fumarate (0.1-0.5%, vol/vol) to a continuous culture of N. salmonicolor growing on acetate caused a rapid decrease in the specific activity of isocitrate lyase in the bacterium, the kinetics being similar to those previously reported when the effect was studied in batch culture. The degree of inhibition was related to the amount of fumarate added. When sodium fumarate (0.5%, vol/vol) was added with cyclic AMP (3 or 30 mM) there was a dramatic but incomplete release of the inhibition of isocitrate lyase synthesis. Dibutyryl cyclic AMP (3 mM) had no significant effect. In all cases, the initial specific activity of isocitrate lyase before any additions was 150-160 nmol min-1 (mg of extract protein)-1 and the growth rate did not change appreciably during the period of the experiment.This publication has 1 reference indexed in Scilit:
- Continuous-flow culture of the ERK and L types of mammalian cellsExperimental Cell Research, 1964