A Statistical Evaluation of a Growth Substance Bioassay Method Using Extracts of Dormant Peach Buds.

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Abstract
Methanol extracts of dormant peach buds were analyzed for possible growth promoting and inhibiting substances. Paper chromatography techniques and the wheat coleoptile straight growth test were used. A growth inhibitor (Rf 0.53) occurred in all peach bud samples. The statistical study revealed that replications of coleoptiles treated similarly is an underestimate of the experimental error. The largest component of experimental error was the failure of known and unknown growth substances chromatographed always to appear in the same section. For the type of experiments described, 1 or 2 coleoptiles per section are indicated as furnishing the greatest information per hour spent.