Low temperature preservation of rabbit embryos

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Abstract
Rabbit embryos (4-cell, 8-cell and morulae) survived freezing and thawing in the presence of 1.5 M-DMSO [dimethylsulfonide]. Highest survival rates, assessed by continued development in vitro for 48-72 h, were obtained with cooling rates of 0.74-0.90.degree. C/min and thawing rates of .apprx. 2 and .apprx. 15.degree. C/min (76%, 83%, 77% and 69%, respectively). Transfer of embryos directly to liquid N after cooling at optimal rates to -110.degree. C instead of -80.degree. C significantly reduced the incidence of rupture of the zona pellucida and mucin coat and increased the rate of recovery. Viability after transfer was low (7% liveborn) and was not correlated with the higher rates of development in vitro to the morula and blastocyst stage.
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