Sequence‐specific hypomethylation of the tobacco genome induced with dihydroxypropyladenine, ethionine and 5‐azacytidine
- 19 October 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 353 (3) , 309-311
- https://doi.org/10.1016/0014-5793(94)01048-x
Abstract
Higher plant DNA is methylated at CG and CNG targets. In this study we have investigated the tobacco methylation system in tissue culture using the methylation inhibitors 5‐azacytidine (5‐azaC), dihydroxypropyladenine (DHPA) and ethionine (Ethi), and methylation‐sensitive restriction endonucleases HpaII, MspI, HhaI, EcoRII, ScrFI, and Fnu4HI. Surprisingly, CAG/CTG sequences, contrary to CG doublets and CCG/CGG triplets, appeared to be refractory to the inhibitory effect of 5‐azaC. Thus 5‐azaC cannot be considered a general inhibitor of DNA methylation in tobacco cells. On the other hand, DHPA, the inhibitor of S‐adenosylhomocysteine (SAH) hydrolase, and Ethi caused hypomethylation of both CAG/CTG and CCG/CGG triplets but not of the CG doublets. The sensitivity of triplet‐specific methylation to the inhibition of SAH hydrolase suggests the possibility that plant‐specific DNA methylation at CNG targets might be modulated by alterations of the SAH/S‐Adenosyhnethionine ratio in plant cells.Keywords
This publication has 17 references indexed in Scilit:
- Characterization of the Nicotiana tabacum L. genome by molecular cytogeneticsMolecular Genetics and Genomics, 1993
- Differential sensitivity of CG and CCG DNA sequences to ethionine‐induced hypomethylation of the Nicotiana tabacum genomeFEBS Letters, 1992
- Isolation and characterization of two middle repetitive DNA sequences of nuclear tobacco genomeTheoretical and Applied Genetics, 1991
- Site-specific methylation: effect on DNA modification methyltransferases and restriction endonucleasesNucleic Acids Research, 1991
- A BamHI family of highly repeated DNA sequences of Nicotiana tabacumTheoretical and Applied Genetics, 1989
- Cloning and sequencing of a cDNA encoding DNA methyltransferase of mouse cellsJournal of Molecular Biology, 1988
- The effect of some 9-substituted adenine derivatives on the development of seedling roots of broad beanBiologia plantarum, 1986
- Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics.Proceedings of the National Academy of Sciences, 1984
- Broad-spectrum antiviral activity of adenosine analoguesAntiviral Research, 1984
- Sequence specificity of methylation in higher plant DNANature, 1981