THE ACETYLATION OF TESTOSTERONE BY RAT AND MOUSE MAMMARY TISSUE

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Abstract
Experiments on the metabolism of [4-14C]testosterone by the mammary gland of pregnant rats and by mammary tumours induced with dimethylbenzanthracene indicated the formation of a minor metabolite with the same chromatographic behaviour as testosterone acetate. A similar compound was found in the mammary gland of pregnant mice and in spontaneous mammary tumours in mice. This note describes the identification of this new metabolite of testosterone. [4-14C]Testosterone (3 μc, specific activity 21·7 μc/μmole) was incubated with mammary tissue slices, weighing 0·5 g., in 2·5 ml. Krebs-Ringer phosphate solution of pH 7·4 for 2 hr. at 37°. The extraction procedure and Girard separation have been described elsewhere (King, Gordon & Helfenstein, 1964). The ketonic fraction was applied to an alumina column (Kellie & Wade, 1957) as modified by King et al. (1964). The radioactive material eluted with 20–45 ml. of 0·15% ethanol in benzene contained mostly androstanedione and androstenedione

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