Pattern of Islet Lysosomal Enzyme Activities and Insulin Secretory Response

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Abstract
The pattern of lysosomal enzyme activities in isolated pancreatic islets was studied in 3 different strains of mice, NMRI, CBA, and C-57, and related to the in vivo insulin release following injection of the insulin secretagogues glucose and carbachol. It was observed that the relative specific activities among the islet enzymes studied did not show the same pattem in the different strains although β-glucuronidase always displayed the lowest activity. Comparison between the strains revealed that acid phosphatase activity was of the same magnitude in all 3 strains. Islet activities of acid amyloglucosidase, β-glucuronidase, and N-acetylglucosaminidase, however, were largest in NMRI, intermediate in CBA, and lowest in C-57. This activity pattern roughly correlated with the insulin secretory response to an intravenous injection of glucose, whereas insulin release induced by the cholinergic agonist carbachol was of similar magnitude in all strains. It is suggested that lysosomes might be involved in certain insulin-releasing processes and that there exists both a quantitative and a qualitative heterogeneity among lysosomal enzymes in the islet tissue of different strains of mice.

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