Glycine-Rich Antifreeze Proteins from Snow Fleas

Abstract
We purified antifreeze proteins from winter-active snow fleas, Hypogastrura harveyi . These 6.5- and 15.7-kilodalton thermolabile proteins are glycine-rich (45% of the residues), and the short isoform is composed of the tripeptide repeat Gly-X-X. This makes them very different from other antifreeze proteins, including two from insects, suggesting independent adaptation to freezing environments.

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