Abstract
Some 2‐alkylthio derivatives of adenosine 5′‐monophosphate (AMP), adenosine 5′‐monophosphorothioate (AMPS) and adenosine 5′‐triphosphate (ATP) were examined as inhibitors of human platelet aggregation. 2‐Methylthio‐AMP, 2‐ethylthio‐AMP, 2‐(pentan‐l‐yl)thio‐AMP, 2‐ethylthio‐AMPS, 2‐methylthio‐ATP and 2‐ethylthio‐ATP (100 μm) each inhibited aggregation induced by adenosine 5′‐diphosphate (ADP) but not by 11α,9α‐epoxymethano prostaglandin H2, a stable endoperoxide analogue. Log dose‐response curves to ADP in the absence and presence of each inhibitor were not parallel and the inhibition could not be overcome by high concentrations of ADP. The ATP analogues achieved greater inhibition of aggregation induced by ADP (5 μm) than did the AMP analogues. The order of potency of the AMP analogues was 2‐ethylthio‐AMPS > 2‐ethylthio‐AMP > 2‐(pentan‐l‐yl)thio‐AMP > 2‐methylthio‐AMP, and 2‐methylthio‐ATP was more potent than 2‐ethylthio‐ATP. These 2‐alkylthio substituted analogues of AMP and ATP are specific but noncompetitive inhibitors of ADP‐induced human platelet aggregation.