Isolation of UV-sensitive clones from a haploid frog cell line
- 1 January 1979
- journal article
- research article
- Published by Springer Nature in Somatic Cell and Molecular Genetics
- Vol. 5 (1) , 117-128
- https://doi.org/10.1007/bf01538790
Abstract
An isolation procedure has been developed which yielded five clones of haploid frog cells which are sensitive to ultraviolet light. This procedure employed a conventional mutagenesis, followed by time for phenotypic expression and then an enrichment for UV-sensitive mutants. The enrichment relies upon the uptake of bromodeoxyuridine (BrdU) by repairing cells following UV-induced damage, rendering repair-proficient cells differentially sensitive to photolysis by black light. The photolysis is potentiated by use of the bisbenzimidazole dye Hoechst 33258. The enriched population was screened for radiation-sensitive isolates resulting in 5 sensitives from 96 tested. No mutants were obtained from 300 isolates tested from a population which had not undergone enrichment.This publication has 13 references indexed in Scilit:
- 33258 Hoechst enhancement of the photosensitivity of bromodeoxyuridine-substituted cellsSomatic Cell and Molecular Genetics, 1976
- Loss of a thermolabile thymidine kinase activity in bromodeoxyuridine resistant (transport deficient, kinase positive) haploid cultured frog cellsExperimental Cell Research, 1976
- XERODERMA PIGMENTOSUM: BIOCHEMICAL AND GENETIC CHARACTERISTICSAnnual Review of Genetics, 1975
- DNA Repair and the Genetic Control of Radiosensitivity in YeastBasic life sciences, 1975
- Specificity and frequency of ultraviolet-induced reversion of an iso-1-cytochrome c ochre mutant in radiation-sensitive strains of yeastJournal of Molecular Biology, 1974
- Origin of thymidine kinase deficient (TK−) haploid frog cells via an intermediate thymidine transport deficient (TT−) phenotypeJournal of Cellular Physiology, 1973
- Genetic Heterogeneity of Xeroderma Pigmentosum demonstrated by Somatic Cell HybridizationNature New Biology, 1972
- Stable Haploid Cultured Cell Lines from frog EmbryosProceedings of the National Academy of Sciences, 1970
- Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells.Proceedings of the National Academy of Sciences, 1968
- Genetics of somatic mammalian cells. V. Treatment with 5-bromodeoxyuridine and visible light for isolation of nutritionally deficient mutants.Proceedings of the National Academy of Sciences, 1967