Effects of Solvent Composition and Temperature on the Normal Phase Liquid Chromatographic Separation of Substituted Phenols

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Abstract
Temperature-dependent measurements of the capacity factors for the liquid chromatographic separations of position isomers of cresol and chlorophenol have been made at various concentrations of 2-propanol in n-heptane using an amino bonded phase column. Extra-thermodynamic relationships, i.e. linear free energy relationship and linear ΔH-pKa relationships are applied to demonstrate that the basic interaction mode does not vary with the solvent composition, consistent with an earlier report by Scott and Kucera. Hydrogen bonding interaction at the molecular level is proposed.