STUDY OF IN VITRO METHODS FOR THE DEMONSTRATION OF ISO-AGGLUTINATION WITH THE BLOODS OF NORMAL AND OF ILL DOGS

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Abstract
Because of observations contradicting the usual concept that distinct iso-agglutination does not occur with normal dog blood and that blood grouping in dogs is unnecessary, a dependable in vitro method for demonstrating iso-agglutination in dog blood was developed. By using serum albumin soln., as a medium for the suspension of saline-washed erythrocytes previously separated at 4[degree]C and rewashed at 37[degree]C or higher and as a diluent for serum perviously exhausted of any interfering auto-agglutinins active at room temp or higher, specific and reproducible agglutinations were obtained with dog blood in macroscopic tests. Agglutination patterns showed that 90% of 40 mongrel dogs could be placed in 1 of 2 major blood groups. 50% of the dogs had erythrocytes which were not specifically agglutinable whereas their sera agglutinated all erythrocytes possessing agglutinable factors. The hemolysis occurring in normal dog blood is nonspecific.

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