Method for Detection and Identification of Multiple Chromosomal Integration Sites in Transgenic Animals Created with Lentivirus
Open Access
- 1 December 2006
- journal article
- Published by Taylor & Francis in BioTechniques
- Vol. 41 (6) , 715-719
- https://doi.org/10.2144/000112289
Abstract
Transgene delivery systems, particularly those involving retroviruses, often result in the integration of multiple copies of the transgene throughout the host genome. Since site-specific silencing of trangenes can occur, it becomes...Keywords
Funding Information
- National Institutes of Health (U42 RR014821 and P40 RR16939)
This publication has 18 references indexed in Scilit:
- Generation of Transgenic Cattle by Lentiviral Gene Transfer into Oocytes1Biology of Reproduction, 2004
- Efficient generation of transgenic pigs using equine infectious anaemia virus (EIAV) derived vectorFEBS Letters, 2004
- Lentivirally generated eGFP‐transgenic rats allow efficient cell tracking in vivoGenesis, 2004
- Efficient transgenesis in farm animals by lentiviral vectorsEMBO Reports, 2003
- Transgenesis by lentiviral vectors: Lack of gene silencing in mammalian embryonic stem cells and preimplantation embryosProceedings of the National Academy of Sciences, 2002
- Germline Transmission and Tissue-Specific Expression of Transgenes Delivered by Lentiviral VectorsScience, 2002
- Chromosomal position effects and the modulation of transgene expressionReproduction, Fertility and Development, 1994
- PCR amplification techniques for chromosome walkingTrends in Biotechnology, 1992
- Genomic walking and sequencing by oligo-cassette mediated polymerase chain reactionNucleic Acids Research, 1990
- Tracking of mouse cell lineage using microinjected DNA sequences: Analyses using genomic Southern blotting and tissue-section in situ hybridizationsDifferentiation, 1987