FtsZ from Divergent Foreign Bacteria Can Function for Cell Division inEscherichia coli
- 1 October 2006
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 188 (20) , 7132-7140
- https://doi.org/10.1128/jb.00647-06
Abstract
FtsZs from Mycoplasma pulmonis (MpuFtsZ) and Bacillus subtilis (BsFtsZ) are only 46% and 53% identical in amino acid sequence to FtsZ from Escherichia coli (EcFtsZ). In the present study we show that MpuFtsZ and BsFtsZ can function for cell division in E. coli provided we make two modifications. First, we replaced their C-terminal tails with that from E. coli, giving the foreign FtsZ the binding site for E. coli FtsA and ZipA. Second, we selected for mutations in the E. coli genome that facilitated division by the foreign FtsZs. These suppressor strains arose at a relatively high frequency of 10(-3) to 10(-5), suggesting that they involve loss-of-function mutations in multigene pathways. These pathways may be negative regulators of FtsZ or structural pathways that facilitate division by slightly defective FtsZ. Related suppressor strains were obtained for EcFtsZ containing certain point mutations or insertions of yellow fluorescent protein. The ability of highly divergent FtsZs to function for division in E. coli is consistent with a two-part mechanism. FtsZ assembles the Z ring, and perhaps generates the constriction force, through self interactions; the downstream division proteins remodel the peptidoglycan wall by interacting with each other and the wall. The C-terminal peptide of FtsZ, which binds FtsA, provides the link between FtsZ assembly and peptidoglycan remodeling.Keywords
This publication has 55 references indexed in Scilit:
- Evidence for functional overlap among multiple bacterial cell division proteins: compensating for the loss of FtsKMolecular Microbiology, 2005
- Diverse Paths to Midcell: Assembly of the Bacterial Cell Division MachineryCurrent Biology, 2005
- SlmA, a Nucleoid-Associated, FtsZ Binding Protein Required for Blocking Septal Ring Assembly over Chromosomes in E. coliMolecular Cell, 2005
- Mutants of FtsZ Targeting the Protofilament Interface: Effects on Cell Division and GTPase ActivityJournal of Bacteriology, 2005
- Cytokinesis in BacteriaMicrobiology and Molecular Biology Reviews, 2003
- Interaction between FtsZ and FtsW of Mycobacterium tuberculosisJournal of Biological Chemistry, 2002
- A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applicationsNature Biotechnology, 2002
- Genetic Analysis of the Escherichia coli FtsZ·ZipA Interaction in the Yeast Two-hybrid SystemJournal of Biological Chemistry, 2001
- A Conserved Residue at the Extreme C-Terminus of FtsZ Is Critical for the FtsA-FtsZ Interaction in Staphylococcus aureusBiochemical and Biophysical Research Communications, 2000
- Human gamma-tubulin functions in fission yeast.The Journal of cell biology, 1994