Comparison of sensitivity of BHK-21 and murine neuroblastoma cells in the isolation of a street strain rabies virus
- 1 August 1987
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 25 (8) , 1456-1458
- https://doi.org/10.1128/jcm.25.8.1456-1458.1987
Abstract
The sensitivities of BHK-21 (C-13) and murine neuroblastoma (C-1300; clone NA) cells for the isolation of small quantities of a street strain rabies virus were compared. Suspension of brain from mice sacrificed prior to the onset of clinical signs of rabies were used to stimulate weakly positive diagnostic specimens. The results of cell culture isolation were compared with those of the direct fluorescent-antibody test and virus isolation in weanling mice. Neuroblastoma cells were more sensitive to the street strain rabies virus than were BHK-21 cells. Neuroblastoma cell virus isolation, the mouse inoculation test, and the fluorescent-antibody test all showed comparable sensitivity.This publication has 10 references indexed in Scilit:
- Rabies serogroup viruses in neuroblastoma cells: propagation, "autointerference," and apparently random back-mutation of attenuated viruses to the virulent stateInfection and Immunity, 1980
- Isolation of Field Rabies Virus Strains in CER and Murine Neuroblastoma Cell CulturesIntervirology, 1977
- RABIES VIRUS-INFECTION IN MOUSE NEUROBLASTOMA-CELLS1977
- Expression of neuronal phenotypes in neuroblastoma cell hybridsDevelopmental Biology, 1974
- Serologically demonstrable cell surface specificities on mouse neuroblastoma C1300.1973
- Laboratory techniques in rabies: the mouse inoculation test.1973