Changes in Cytochemistry of Sensory and Nonsensory Cells in Gentamicin-Treated Cochleas
Open Access
- 1 June 2003
- journal article
- Published by Springer Nature in Journal of the Association for Research in Otolaryngology
- Vol. 4 (2) , 196-218
- https://doi.org/10.1007/s10162-002-2037-7
Abstract
Effects of a single local dose of gentamicin upon sensory and nonsensory cells throughout the cochlea were assessed by changes in immunostaining patterns for a broad array of functionally important proteins. Cytochemical changes in hair cells, spiral ganglion cells, and cells of the stria vascularis, spiral ligament, and spiral limbus were found beginning 4 days post administration. The extent of changes in immunostaining varied with survival time and with cell type and was not always commensurate with the degree to which individual cell types accumulated gentamicin. Outer hair cells, types I and II fibrocytes of the spiral ligament, and fibrocytes in the spiral limbus showed marked decreases in immunostaining for a number of constituents. In contrast, inner hair cells, type III fibrocytes and root cells of the spiral ligament, cells of the stria vascularis, and interdental cells in the spiral limbus showed less dramatic decreases, and in some cases they showed increases in immunostaining. Results indicate that, in addition to damaging sensory cells, local application of gentamicin results in widespread and disparate disruptions of a variety of cochlear cell types. Only in the case of ganglion cells was it apparent that the changes in nonsensory cells were secondary to loss or damage of hair cells. These results indicate that malfunction of the ear following gentamicin treatment is widespread and far more complex than simple loss of sensory elements. The results have implications for efforts directed toward detecting, preventing, and treating toxic effects of aminoglycosides upon the inner ear.Keywords
This publication has 62 references indexed in Scilit:
- Overexpression of Copper/Zinc- Superoxide Dismutase Protects from Kanamycin-Induced Hearing LossAudiology and Neurotology, 2001
- Human diseases: clues to cracking the connexin code?Trends in Cell Biology, 2001
- Protection from ototoxicity of intraperitoneal gentamicin in guinea pigKidney International, 2000
- Gap junction systems in the mammalian cochleaBrain Research Reviews, 2000
- A Point Mutation in a Plasma Membrane Ca2+-ATPase Gene Causes Deafness in Wriggle Mouse SagamiBiochemical and Biophysical Research Communications, 1999
- Alternatively Spliced Isoforms of the Na+/Ca2+Exchanger in the Guinea Pig CochleaBiochemical and Biophysical Research Communications, 1997
- Co-expression of glial fabrillary acidic protein and vimentin in reactive astrocytes following brain injury in ratsBrain Research, 1991
- Immunohistochemical localization of vimentin in the gerbil inner ear.Journal of Histochemistry & Cytochemistry, 1989
- Distribution of immunoreactive Na+,K+-ATPase in gerbil cochlea.Journal of Histochemistry & Cytochemistry, 1989
- Calcium transport and buffering in neuronsTrends in Neurosciences, 1988